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KMID : 1138520030060020045
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Journal of Pharmacopuncture
2003 Volume.6 No. 2 p.45 ~ p.56
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The Effects of Bee Venom on iNOS, TNF-{alpha} and NF-kB in RAW 264.7 Cells
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Kim Goon-Joong
Sim Sung-Yong
Lee Seong-No
Kim Kee-Hyun
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Abstract
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Objective : The purpose of this study was to investigate the effects of Bee Venom on the lipopolysaccharide(LPS), sodium nitroprusside(SNP), hydrogen peroxide(H_2O_2)-induced expression inducilble nitric oxide synthetase(iNOS), tumor necrosis factor-{alpha}(TNF-{alpha}) and nuclear factor kappa B(NF-kB) in RAW 264.7 cells, a murine macrophage cell line.
Method : The expressions of expression iNOS and TNF-{alpha} were determined by western blotting with corresponding antibodies. The expressions of expression NF-kB was assayed by EMSA method.
Results : 1. The 0.5, 1 and 5{mu}g/mg of bee venom on LPS-induced expression of iNOS, the 5{mu}g/mg of bee venom on SNP-induced expression of iNOS and the 1{mu}g/mg of bee venom on H_2O_2-induced expression of iNOS compared with control were inhibited significantly. 2. The 0.5, 1 and 5{mu}g/mg of bee venom inhibited significantly LPS and H_2O_2-induced expression of TNF-{alpha} compared with control, respectively. The 0.5{mu}g/mg of bee venom increased significantly SNP-induced expression of TNF-{alpha} compared with control. 3. The 5{mu}g/mg of bee venom on LPS-induced expression of NF-kB, the 0.5{mu}g/mg of bee venom on SNP-induced expression of NF-kB and the 0.5, 5{mu}g/mg of bee venom on H_2O_2-induced expression of NF-kB were inhibited significantly compared with control, respectively.
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KEYWORD
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Bee Venom, iNOS, $TNF-{\alpha}$, NF-kB, RAW 264.7 cell
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